Glucokinase from Bacillus stearothermophilus is widely used in biochemical research and industrial applications due to its thermostability and enzymatic activity. It plays a crucial role in glucose metabolism studies, particularly in understanding glucose phosphorylation processes. In biotechnology, it is employed in biosensors for glucose detection, offering high specificity and stability under varying conditions. Additionally, it is utilized in the production of high-fructose corn syrup, where it aids in the conversion of glucose to fructose. Its robustness at elevated temperatures makes it valuable in industrial enzymatic processes requiring durability and efficiency.