UV-VIS Anti-Hyaluronidase Assay
- Product Code: 31886
Spectrophotometric determination of hyaluronidase inhibition by measuring absorbance increase at 232 nm from unsaturated disaccharide products of hyaluronic acid hydrolysis.
description Sample Requirement
solid extract or powder · 0.05 · g
insert_drive_file Report of Previously Tested Sample
timeline Service Steps
| Step | Procedure | Expected Result |
|---|---|---|
|
1
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Weigh 0.01... |
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sample solution |
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2
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The reaction... |
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sample and enzyme mixture |
|
3
|
Incubate at... |
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pre-incubation |
|
4
|
Add 100... |
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sample, enzyme and substrate mixture |
|
5
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Incubate at... |
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enzyme-substrate reaction |
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6
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The reaction... |
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stop reaction |
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7
|
Absorbance is... |
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absorbance of sample |
|
8
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Each measurement... |
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ensure test protocol |
|
9
|
The percentage... |
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% inhibition |
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