HSL-IN-1 is primarily utilized in research settings to study the role of hormone-sensitive lipase (HSL) in metabolic processes. It is a potent inhibitor of HSL, making it a valuable tool for investigating lipid metabolism, particularly in adipose tissue. Researchers use HSL-IN-1 to explore its effects on fat breakdown and storage, which can provide insights into conditions like obesity, diabetes, and metabolic syndrome. Additionally, it aids in understanding the regulation of fatty acid release and energy homeostasis, contributing to the development of potential therapeutic strategies for metabolic disorders. Its application is mainly confined to preclinical studies, focusing on cellular and animal models to elucidate HSL's physiological and pathological roles.