Used in biomedical research to measure diglyceride (DAG) levels in human biological samples such as serum, plasma, or tissue extracts. DAG is a key lipid signaling molecule involved in metabolic pathways and cellular regulation, including insulin signaling and fat metabolism. This ELISA kit enables researchers to study conditions related to metabolic disorders, obesity, diabetes, and non-alcoholic fatty liver disease (NAFLD). It provides a sensitive and specific method for quantifying glycerol difatty esters, supporting studies on lipid metabolism and energy homeostasis in clinical and experimental settings.