Used in research to measure levels of 6-keto-PGF1α, a stable metabolite of prostacyclin, in biological samples such as plasma, serum, and urine. This assay helps assess endothelial function and vascular health in studies related to cardiovascular diseases, inflammation, and thrombosis. Commonly applied in preclinical research involving animal models, especially rats, to evaluate the impact of drugs, oxidative stress, or disease states on prostaglandin metabolism. The kit enables high-throughput, sensitive, and specific quantification, supporting investigations in pharmacology, physiology, and pathophysiology.