Used in biochemical research to quantify intramuscular fat levels in mouse models, particularly in studies related to metabolism, obesity, diabetes, and muscle physiology. The ELISA kit enables researchers to measure specific biomarkers associated with fat deposition within muscle tissue, providing insights into metabolic health and the effects of pharmaceuticals, diet, or genetic modifications. It is widely applied in preclinical studies to assess interventions targeting insulin resistance, sarcopenia, and lipid metabolism disorders. The assay offers high sensitivity and specificity, making it suitable for detecting small changes in IMF-related proteins or metabolites in serum or tissue samples.