Used in research settings to quantify direct (conjugated) bilirubin levels in human biological samples such as serum or plasma. This ELISA kit enables accurate measurement of bilirubin for studies related to liver function, bile duct disorders, and jaundice. It is particularly useful in clinical research investigating hepatobiliary diseases, neonatal jaundice, and metabolic disorders. The assay supports high-throughput analysis with high sensitivity and specificity, making it suitable for diagnostic development and biomarker validation in liver and gallbladder pathologies.