Cell lysis buffer is widely used in molecular biology and biochemistry for breaking open cells to extract cellular components such as DNA, RNA, proteins, and organelles. This formulation is specifically designed for Western blotting and immunoprecipitation (IP) applications and does not contain protease inhibitors. It is essential in experiments involving protein purification, gene expression analysis, and enzyme activity studies. The buffer helps to disrupt cell membranes while maintaining the integrity of the molecules of interest. It is commonly employed in techniques like Western blotting, PCR, and immunoprecipitation. Additionally, it is used in diagnostic labs for sample preparation and in research settings to study cellular mechanisms and pathways. The buffer’s effectiveness makes it a critical tool in both academic and industrial laboratories.