ISO-1 is primarily used in research settings to study its role as an inhibitor of macrophage migration inhibitory factor (MIF). This application is crucial in understanding inflammatory processes, as MIF is a key cytokine involved in immune responses. By inhibiting MIF, ISO-1 helps researchers explore potential treatments for inflammatory diseases, autoimmune disorders, and certain cancers. Additionally, its use in experimental models provides insights into the mechanisms of cell migration and immune regulation, contributing to the development of novel therapeutic strategies.