Monodispersed magnetite microspheres are widely used in biomedical applications due to their unique magnetic properties. They are employed in magnetic resonance imaging (MRI) as contrast agents to enhance the visibility of internal body structures. These microspheres are also utilized in targeted drug delivery systems, where they can be guided to specific sites in the body using an external magnetic field, ensuring precise treatment with minimal side effects. Additionally, they play a crucial role in magnetic hyperthermia, a cancer treatment method where the microspheres generate heat under an alternating magnetic field to destroy tumor cells. In the field of diagnostics, they are used in immunoassays and cell separation techniques, enabling efficient isolation and analysis of specific cell types or biomolecules. Their uniform size and magnetic responsiveness make them valuable in various research and industrial applications, including environmental remediation for the removal of contaminants from water.