Monodispersed magnetite microspheres are widely used in biomedical applications due to their magnetic properties and uniform size. They are employed in magnetic resonance imaging (MRI) as contrast agents to enhance image clarity and improve diagnostic accuracy. In drug delivery systems, these microspheres enable targeted therapy by guiding drugs to specific sites in the body using external magnetic fields, reducing side effects and increasing treatment efficiency. Additionally, they are utilized in cell separation and purification processes, where their magnetic nature allows for the isolation of specific cell types from complex mixtures. In environmental applications, they are used for wastewater treatment to remove heavy metals and other pollutants through magnetic separation techniques. Their consistent size and magnetic responsiveness make them valuable in various scientific and industrial fields.