Monodispersed magnetite microspheres are widely used in biomedical applications due to their magnetic properties and uniform size. They are employed in magnetic resonance imaging (MRI) as contrast agents to enhance image quality by improving the visibility of internal structures. In drug delivery systems, these microspheres serve as carriers for targeted therapy, allowing precise delivery of drugs to specific sites in the body using external magnetic fields. They are also utilized in cell separation and sorting processes, where their magnetic nature enables efficient isolation of specific cell types from complex mixtures. Additionally, these microspheres play a role in hyperthermia treatment for cancer, where they generate heat under an alternating magnetic field to destroy cancer cells. Their application extends to environmental remediation, where they are used for the removal of pollutants from water through magnetic separation techniques.