Monodispersed magnetite microspheres are widely used in biomedical applications, particularly in magnetic resonance imaging (MRI) as contrast agents to enhance image clarity. They are also employed in targeted drug delivery systems, where their magnetic properties allow for precise control and localization of therapeutic agents to specific areas of the body. In addition, these microspheres are utilized in cell separation and sorting processes, enabling the isolation of specific cell types for research and diagnostic purposes. Their uniform size and magnetic responsiveness make them valuable in environmental applications, such as the removal of contaminants from water through magnetic separation techniques. Furthermore, they play a role in the development of biosensors, improving the sensitivity and accuracy of detecting biological molecules.