This compound is primarily utilized in the field of bioconjugation and protein modification. It serves as a versatile linker for attaching various molecules, such as peptides, proteins, or antibodies, to other functional groups or surfaces. Its reactive pyrrole dione moiety enables efficient coupling with thiol groups, making it valuable in the development of targeted drug delivery systems, diagnostic probes, and bioconjugate vaccines. Additionally, it is employed in the synthesis of complex biomolecules for research in biochemistry and molecular biology, particularly in studies involving protein-protein interactions and cellular labeling. Its design allows for precise control over conjugation, ensuring minimal disruption to the biological activity of the molecules involved.