Used as a heterobifunctional crosslinker in bioconjugation, it facilitates the attachment of proteins, peptides, or other biomolecules to surfaces or other molecules. The maleimide group reacts with thiols (-SH) in cysteine residues, while the NHS ester group reacts with primary amines (-NH2), enabling precise and stable conjugation. It is particularly useful in antibody-drug conjugates (ADCs), where it links antibodies to cytotoxic drugs for targeted cancer therapy. Additionally, it is employed in immobilizing biomolecules on solid supports for diagnostic assays, biosensors, and research applications. Its stability and efficiency make it a valuable tool in biochemistry and biopharmaceutical development.