Used in bioconjugation and protein modification, it serves as a linker molecule to attach functional groups or therapeutic agents to biomolecules. Its t-butyl ester group provides stability, while the maleimide moiety allows for selective conjugation with thiol groups in peptides, proteins, or antibodies. This makes it valuable in drug delivery systems, antibody-drug conjugates, and targeted therapies. It is also employed in research for labeling and tracking biomolecules in cellular studies. The PEG spacer enhances solubility and reduces steric hindrance, improving the efficiency of conjugation processes.