The CoenzymeⅠ NAD(H) Content Assay Kit is widely used in biochemical and biomedical research to measure the levels of NAD+ and NADH in various biological samples. It is essential for studying cellular metabolism, particularly in pathways like glycolysis, the tricarboxylic acid cycle, and oxidative phosphorylation. Researchers use this kit to investigate the redox state of cells, which is critical for understanding processes such as energy production, aging, and stress responses. It is also applied in cancer research to explore how altered NAD+ levels contribute to tumor metabolism. Additionally, the kit is utilized in drug discovery to evaluate the efficacy of compounds targeting NAD+-dependent enzymes, such as sirtuins and PARPs. Its high sensitivity and specificity make it a valuable tool for advancing studies in metabolic diseases, mitochondrial function, and cellular health.