Used in research settings to detect and quantify levels of azidothymidine (AZT) metabolites in biological samples. Commonly applied in pharmacokinetic studies to monitor drug metabolism and clearance in preclinical trials involving mice. Supports evaluation of antiretroviral drug distribution and efficacy in animal models of HIV infection. Enables high-throughput screening of AZT concentration in serum, plasma, or tissue homogenates with high sensitivity and specificity. Widely used in immunology and virology labs for assessing therapeutic drug monitoring and toxicity profiles in rodent studies.