Used in biomedical research to quantify prostaglandin E metabolites in biological samples such as urine, plasma, or cell culture supernatants. Enables assessment of systemic inflammation and COX pathway activity, particularly in studies related to asthma, cardiovascular diseases, and inflammatory disorders. Supports high-throughput screening in clinical and preclinical settings due to its sensitivity and specificity. Commonly applied in pharmacological studies evaluating the effects of NSAIDs or other anti-inflammatory agents. Provides a reliable tool for monitoring eicosanoid metabolism without the need for complex instrumentation like mass spectrometry.