Used in biomedical research to measure cyclic guanosine monophosphate (cGMP) levels in human samples such as plasma, serum, or tissue extracts. This assay helps study cellular signaling pathways involving nitric oxide and cGMP, which play key roles in cardiovascular function, neurotransmission, and inflammation. Commonly applied in studies of erectile dysfunction, pulmonary hypertension, and the effects of drugs like PDE5 inhibitors. The kit enables high-throughput, quantitative analysis in clinical and research laboratories.