Used in research settings to detect and quantify iron-containing porphyrin complexes, particularly in biological samples such as blood, plasma, or tissue extracts. Commonly applied in studies related to heme metabolism, oxidative stress, and disorders like porphyria or hemolytic diseases. The kit enables high-throughput analysis using enzyme-linked immunosorbent assay (ELISA) methodology, providing sensitive and specific measurement of ironporphyrin levels. Widely utilized in biomedical research, toxicology, and drug development to monitor heme-derived biomarkers and assess cellular damage due to free radical activity.