Used in biomedical research to quantify trimethylamine N-oxide (TMAO) levels in biological samples such as serum, plasma, and urine. It plays a critical role in studies linking TMAO to cardiovascular diseases, chronic kidney disease, and metabolic disorders. The kit enables high-throughput analysis in clinical and preclinical settings, supporting investigations into gut microbiota metabolism, dietary influences on health, and development of diagnostic markers. Widely applied in epidemiological studies and drug development to assess TMAO as a potential biomarker for disease risk and progression.