ONO-RS-082 is a potent and selective inhibitor of cytosolic phospholipase A2 (cPLA2), particularly cPLA2α (IC₅₀ = 0.11 μM), with much lower potency against secretory PLA2 isoforms. It is primarily used in research to block arachidonic acid release and inhibit eicosanoid biosynthesis. Key applications include studies on inflammation, pain signaling, neuroinflammation, cancer cell invasion/migration, and PLA2-mediated neuronal signaling. Researchers utilize it in biochemical assays, cell-based experiments, and animal models to investigate the role of cPLA2 in physiological and pathological processes, aiding in the development of anti-inflammatory and neuroprotective therapies.