Used as a defined minimal growth medium for cultivating yeast, particularly Saccharomyces cerevisiae. Supports controlled studies of yeast metabolism, gene expression, and nutrient requirements. Commonly used in molecular biology and genetics research to maintain selective pressure when working with plasmids carrying nutritional markers. Can be supplemented with specific amino acids, nucleic acid precursors, or carbon sources to study metabolic pathways or gene function. Ideal for high-throughput screening, fermentation studies, and protein expression experiments where precise control of nutrients is required.