Antimicrobial Preservative Effectiveness Challenge Test against Escherichia coli

  • Product Code: 253897
Microbial Challenge Test for testing the effectiveness of preservatives used in formulas. To ensure that it is effective enough to control infection using ISO 11930.
฿2,490.00

label Sample Names

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assignment Instructions

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description Sample Requirement

100 g

insert_drive_file Report of Previously Tested Sample

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timeline Service Steps

Step Procedure Expected Result
1

Tryptic Soy...

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Preparation of the media

2

Bacteria: Escherichia...

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Test Microorganism

3

Prepare a...

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Preparation of the inoculum (N)

4
  • Sampling: Dispense...
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Day 0: Inoculation & Calculation of N0

5

At each...

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Day 7, 14, and 28: Sampling & Enumeration

6

Incubate plates:...

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Colony counts for log reduction calculation.

7

To evaluate...

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Calculation of Log Reduction (Rx​)

8

At 0,...

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Log10 reductions: Criteria A (no increase 7d fungi/bact, ≥3log 14d) or B (≤0.5log 7d, ≥3log 28d).

9

Include sterility...

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Controls pass (no growth in sterility, recovery >70% in neutralizer).

Antimicrobial Preservative Effectiveness Challenge Test against Escherichia coli
Microbial Challenge Test for testing the effectiveness of preservatives used in formulas. To ensure that it is effective enough to control infection using ISO 11930. Sample Requirement: 100 g
Microbial Challenge Test for testing the effectiveness of preservatives used in formulas. To ensure that it is effective enough to control infection using ISO 11930. Sample Requirement: 100 g
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Service Steps
Step Procedure Expected Result
1

Tryptic Soy...

Preparation of the media

2

Bacteria: Escherichia...

Test Microorganism

3

Prepare a...

Preparation of the inoculum (N)

4
  • Sampling: Dispense...

Day 0: Inoculation & Calculation of N0

5

At each...

Day 7, 14, and 28: Sampling & Enumeration

6

Incubate plates:...

Colony counts for log reduction calculation.

7

To evaluate...

Calculation of Log Reduction (Rx​)

8

At 0,...

Log10 reductions: Criteria A (no increase 7d fungi/bact, ≥3log 14d) or B (≤0.5log 7d, ≥3log 28d).

9

Include sterility...

Controls pass (no growth in sterility, recovery >70% in neutralizer).