Disk Diffusion Assay for Antimicrobial Activity against Escherichia coli
- Product Code: 35523
Agar-based method measuring inhibition zone diameters around disks impregnated with test substance on E. coli lawn to assess antimicrobial activity.
description Sample Requirement
Test substance solution 10 mL
insert_drive_file Report of Previously Tested Sample
timeline Service Steps
| Step | Procedure | Expected Result |
|---|---|---|
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1
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Prepare Mueller-Hinton... |
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Solidified agar plates free of cracks or bubbles.
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2
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Inoculate E.... |
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Turbid suspension matching 0.5 McFarland turbidity.
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3
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Dip sterile... |
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Uniform bacterial lawn after drying 3-5 min.
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4
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Place 3-6... |
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Disks absorbed solution without overflow. |
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5
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Invert plates;... |
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Visible growth on lawn.
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6
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Examine plates;... |
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Inhibition zones if antimicrobial activity present; zone > solvent control indicates activity.
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