Tubulysin D is primarily utilized in the field of cancer research due to its potent antitumor properties. It functions by disrupting microtubule dynamics, which are essential for cell division, thereby inhibiting the proliferation of cancer cells. This makes it a valuable compound for studying mechanisms of cell cycle arrest and apoptosis in malignant cells. Researchers are exploring its potential in developing targeted cancer therapies, particularly for drug-resistant tumors. Additionally, Tubulysin D is being investigated as a payload in antibody-drug conjugates (ADCs), where it can be delivered specifically to cancer cells, minimizing damage to healthy tissues. Its high cytotoxicity underscores its promise as a potent agent in oncology drug development.