Used in biomedical research to quantify levels of mitochondrial respiratory chain complex I in mouse tissue samples, particularly in studies related to mitochondrial dysfunction, neurodegenerative diseases, aging, and metabolic disorders. Enables researchers to assess mitochondrial health and oxidative phosphorylation efficiency in experimental models. Commonly applied in preclinical studies involving brain, muscle, and liver tissues where mitochondrial activity is critical. The ELISA format allows for high-throughput, specific, and sensitive detection, making it valuable for evaluating disease progression or therapeutic interventions targeting mitochondrial function.