Z-Dehydro-Ala-OMe is primarily utilized in peptide synthesis and organic chemistry research. It serves as a building block for creating modified peptides, enabling the study of peptide structure and function. Its dehydroalanine moiety is particularly valuable for introducing double bonds into peptide chains, which can influence conformational stability and reactivity. This compound is also employed in the development of peptidomimetics, aiding in the design of molecules that mimic natural peptides for therapeutic applications. Additionally, it finds use in the synthesis of bioactive compounds and as a tool for investigating enzyme mechanisms and protein interactions.