mTOR inhibitor-3 is primarily used in biochemical research to study the mTOR signaling pathway, which plays a critical role in cell growth, proliferation, and survival. It is employed to inhibit mTOR activity, allowing scientists to investigate its effects on various cellular processes, including autophagy, metabolism, and cancer progression. This compound is particularly valuable in preclinical studies to understand the therapeutic potential of mTOR inhibition in diseases such as cancer, neurodegenerative disorders, and metabolic conditions. Additionally, it aids in the development of targeted therapies by providing insights into the molecular mechanisms regulated by mTOR.