Spastazoline is primarily used in research settings to study the role of specific receptors in the nervous system. It acts as a selective agonist for certain subtypes of muscarinic acetylcholine receptors, particularly the M4 subtype. This makes it a valuable tool for investigating the physiological and pathological functions of these receptors in the brain and peripheral tissues. Researchers utilize Spastazoline to explore its potential effects on neural pathways, muscle activity, and responses in various disease models, particularly those related to neurological disorders. Its application helps in understanding receptor mechanisms and developing targeted therapeutic strategies.