4-hydroxy Alternariol is primarily studied for its role in mycotoxin research, as it is a metabolite of Alternaria fungi. It is used in scientific studies to understand the toxicological effects of mycotoxins on human health, particularly in relation to food safety and contamination. Researchers analyze its presence in agricultural products to assess potential risks and develop strategies for mitigation. Additionally, it serves as a reference compound in analytical methods, such as chromatography and mass spectrometry, to detect and quantify Alternaria toxins in various samples. Its application is crucial in advancing knowledge about fungal metabolites and their impact on food quality and safety.