(-)-Blebbistatin is widely used in biological research as a selective inhibitor of myosin II, a motor protein involved in muscle contraction and various cellular processes. Its primary application is in studying the role of myosin II in cell motility, cytokinesis, and cytoskeletal dynamics. Researchers use it to inhibit myosin II activity in experiments, allowing them to observe its effects on cell division, migration, and tissue development. Additionally, it is employed in microscopy studies to reduce phototoxicity and improve imaging of live cells. Its ability to reversibly inhibit myosin II makes it a valuable tool in understanding cellular mechanics and developing potential therapeutic strategies for diseases involving myosin dysfunction.