Diosbulbin J is primarily studied for its potential hepatotoxic effects, making it a focus in toxicological research related to liver injury. It is used as a reference compound to investigate mechanisms of drug-induced liver damage, particularly in understanding how certain natural products can lead to hepatocyte apoptosis and oxidative stress. Due to its structural characteristics, it also serves as a model substance in metabolic stability studies and drug metabolism screening, especially in herbal medicine safety evaluation. Research applications include in vitro and in vivo models to assess detoxification strategies and protective agents against liver toxicity.