NEP-IN-2 is primarily utilized in biochemical research as a potent and selective inhibitor of neprilysin (NEP), an enzyme involved in the degradation of various peptides, including natriuretic peptides and amyloid-beta. Its application is significant in studies focusing on cardiovascular diseases, where NEP inhibition can enhance the levels of beneficial peptides that regulate blood pressure and fluid balance. Additionally, it is used in neuroscience research to investigate the role of NEP in amyloid-beta metabolism, which is relevant to understanding Alzheimer's disease pathology. By inhibiting NEP, researchers can explore potential therapeutic strategies for conditions linked to peptide dysregulation.