NEP-In-1 is primarily utilized in biochemical research as a potent and selective inhibitor of neprilysin (NEP), an enzyme involved in the degradation of various bioactive peptides. Its application is significant in studies focused on understanding the role of NEP in physiological and pathological processes, such as cardiovascular regulation, neuropeptide metabolism, and pain modulation. Researchers employ NEP-In-1 to explore its potential therapeutic effects in conditions like hypertension, heart failure, and Alzheimer’s disease, where NEP activity plays a critical role. Additionally, it is used in drug discovery efforts to develop novel treatments targeting NEP-related pathways.