Used in scientific research to study the function of muscarinic acetylcholine receptors, particularly the M4 subtype. Helps in understanding neurological pathways involved in conditions like schizophrenia, Parkinson’s disease, and substance abuse. Acts as a positive allosteric modulator, enhancing receptor response to acetylcholine without directly activating it. Enables targeted investigation of cognitive, motor, and reward-related behaviors in preclinical models. Supports development of selective drugs with fewer side effects compared to traditional orthosteric agents.