Used in neuroscience and clinical research to quantify neuron-specific enolase (NSE) levels in biological samples such as serum, plasma, cerebrospinal fluid, and tissue homogenates. Widely applied in studies related to neuronal damage, including traumatic brain injury, stroke, hypoxic-ischemic encephalopathy, and neurodegenerative diseases. Helps assess the extent of neuronal injury and monitor disease progression or response to treatment. Commonly used in biomarker validation, pharmaceutical research, and diagnostic development due to its high sensitivity and specificity for neuronal tissue.