Used in neuroscience research to quantify levels of nerve growth factor (NGF) in biological samples such as serum, cerebrospinal fluid, and tissue homogenates. Enables investigation of neurodegenerative diseases including Alzheimer's, Parkinson's, and multiple sclerosis by measuring NGF concentration changes associated with disease progression or treatment response. Supports studies on neural development, regeneration, and plasticity in both central and peripheral nervous systems. Commonly applied in preclinical drug development to assess the neurotrophic effects of therapeutic compounds. Provides a reliable, sensitive, and high-throughput method for detecting NGF in cell culture supernatants and animal models.