Used in neuroscience and sleep disorder research to measure orexin protein levels in mouse biological samples such as cerebrospinal fluid, brain tissue homogenates, and serum. Helps investigate the role of orexin in regulating sleep-wake cycles, particularly in models of narcolepsy, where orexin deficiency is a key factor. Enables researchers to evaluate changes in orexin expression under various experimental conditions, including drug treatments, genetic modifications, and circadian rhythm studies. Supports high-throughput screening and quantitative analysis in preclinical studies focused on sleep, addiction, appetite, and energy homeostasis.