Used in neuroscience and pharmacological research to quantify expression levels of orexin receptor 1 in rat tissue samples, particularly in studies related to sleep-wake regulation, narcolepsy, appetite control, and addiction. Enables researchers to assess receptor activity in response to experimental treatments or disease models. Commonly applied in preclinical studies involving rodent models to investigate the role of the orexin system in behavioral and physiological processes. Provides a reliable, high-throughput method for detecting receptor protein concentration in cell lysates or membrane preparations.