Neuropeptide FF trifluoroacetate salt (NPFF) is primarily utilized in research settings to study its role in the central nervous system, particularly in modulating pain perception and opioid tolerance. It is often employed in preclinical studies to investigate its effects on neuropathic pain and its interaction with opioid receptors, providing insights into potential therapeutic applications for pain management. Additionally, NPFF is used to explore its influence on feeding behavior and energy homeostasis, contributing to research on obesity and metabolic disorders. Its ability to modulate neurotransmitter release and neuronal activity makes it a valuable tool in neuroscience research, particularly in understanding the mechanisms underlying addiction and withdrawal.