Used in biomedical research to detect and quantify calcium-binding protein levels in rat tissue samples, particularly in studies related to neurobiology and muscle function. Helps researchers investigate calcium signaling pathways, neuronal damage, and neurodegenerative diseases. Commonly applied in preclinical studies involving brain, spinal cord, and muscle tissues to assess cellular stress, injury, or disease progression. The kit enables high-throughput analysis in laboratory settings, supporting accurate measurement of protein concentration in complex biological samples.