Used in neuroscience research to quantify netrin-1 protein levels in biological samples such as cell lysates, tissue homogenates, and cerebrospinal fluid. Enables investigation of axon guidance mechanisms during neural development and regeneration. Applied in studies of brain development, spinal cord injury, and neurodegenerative diseases. Supports research on synaptic plasticity and neural circuit formation by providing a reliable method to measure netrin-1 concentration in experimental models.