Used in neuroscience and stem cell research to detect and quantify nestin, a protein marker associated with neural stem and progenitor cells. Helps assess cell differentiation, neurogenesis, and brain development in experimental models. Widely applied in studies of brain injury, neurodegenerative diseases, and cancer research, particularly in identifying tumor stem cells in gliomas. Provides a reliable, sensitive method for measuring nestin levels in cell lysates and tissue samples using ELISA technique.