Used in biomedical research to quantify Substance P levels in biological samples such as plasma, serum, cerebrospinal fluid, and tissue homogenates. Widely applied in studies related to neuroinflammation, pain pathways, and neurological disorders including migraine, depression, and multiple sclerosis. Also utilized in pharmacological research to evaluate the effectiveness of drugs targeting tachykinin receptors or neurokinin release. The kit enables high-throughput, sensitive, and specific detection, making it valuable in both academic and clinical research settings for understanding the role of Substance P in immune response, stress, and chronic inflammatory conditions.