Used in biomedical research to measure corticotropin-releasing factor (CRF) levels in rat biological samples such as plasma, serum, or tissue homogenates. This ELISA kit enables researchers to study the hypothalamic-pituitary-adrenal (HPA) axis activity, which plays a central role in the body's response to stress. It is widely applied in neuroscience, endocrinology, and pharmacology studies to investigate stress-related disorders, anxiety, depression, and the effects of drugs on hormonal regulation. The assay provides a sensitive and specific quantitative measurement, supporting both academic research and drug development.