Used in research settings as a metabolite reference for nicotine metabolism studies. It helps in understanding the oxidative pathways of nicotine in biological systems, particularly in liver enzyme activity assessments. Also utilized in analytical chemistry for detection and quantification of nicotine derivatives in biological samples such as urine and plasma. Shows lower biological activity compared to nicotine, making it useful in control experiments for receptor binding studies. Investigated for potential use in developing reduced-toxicity nicotine delivery systems.