LY83583 is primarily used in research settings to study cellular signaling pathways, particularly those involving cyclic guanosine monophosphate (cGMP). It is known to inhibit guanylate cyclase, an enzyme responsible for the synthesis of cGMP, making it a valuable tool for investigating the role of cGMP in various physiological processes. Researchers use it to explore its effects on vascular tone, smooth muscle relaxation, and neuronal signaling. Additionally, LY83583 has been employed in studies related to cardiovascular diseases, pulmonary hypertension, and neurodegenerative disorders, helping to elucidate the mechanisms underlying these conditions. Its ability to modulate cGMP levels also makes it useful in studying cellular responses to nitric oxide and other signaling molecules.