Used extensively in metabolic research and studies involving nitrogen tracing, L-Isoleucine-15N helps in understanding protein synthesis and amino acid metabolism. It is particularly valuable in isotopic labeling experiments, where it aids in tracking the incorporation of nitrogen into proteins and other biomolecules. This compound is also employed in nutritional studies to investigate the role of essential amino acids in diet and health. Additionally, it finds application in the development of diagnostic tools and therapeutic agents, especially in fields focused on metabolic disorders and muscle-related conditions. Its stable isotope nature makes it a crucial tool in NMR spectroscopy and mass spectrometry for precise analytical measurements.